Check your local public health requirements, which may require submission of isolates to a public health laboratory. The American Medical Association Current Procedural Terminology (CPT) codes published in ARUP's Laboratory Test Directory are provided for informational purposes only. Cary Blair media is ideal for transport, and the selective thiosulfate–citrate–bile salts agar (TCBS) is ideal for isolation and identification. Although management of patients with acute watery diarrhea is similar regardless of etiology, public health implications, epidemic spread, and management of cholera outbreaks are distinct, given that V. choleraeis highly virulent and has tremendous epidemic potential. V. cholerae is a facultative anaerobe, and can undergo respiratory and fermentative metabolism. Vibrio cholerae growing on thiosulphate citrate bile salt sucrose (TCBS) agar plates. However, the sensitivity and specificity of this test is not optimal. May also include abnormal ranges. Suggests clinical disorders or settings where the test may be helpful. Synonyms. A medium for the culture of pathogenic Vibrio species as well as Salmonella and Shigella. Check your local public health requirements, which may require submission of isolates to a public health laboratory. Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test.

Multiple specimens (more than one in 24 hours). Standard safety practices in the microbiology laboratory 2. Determining whether Vibrio species may be the cause of diarrhea, This test is generally not useful for patients that have been hospitalized for more than 3 days because the yield from these patients' specimens is very low, as is the likelihood of identifying a pathogen that has not been detected previously. In areas with limited or no laboratory testing, the Crystal® VC dipstick rapid test can provide an early warning to public health officials that an outbreak of cholera is occurring. Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website. You will be subject to the destination website's privacy policy when you follow the link. Click here for your pricing. Other names that describe the test. It is almost impossible to distinguish a single patient with cholera from a patient infected by another pathogen that causes acute watery diarrhea without testing a stool sample. Although rapid diagnost… Buffered glycerol saline is an unacceptable transport media for Vibrio culture. May include related or preferred tests. A review of clinical features of multiple patients who are part of a suspected outbreak of acute watery diarrhea can be helpful in identifying cholera because of the rapid spread of the disease. All Rights Reserved. CDC twenty four seven. Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Pillai DR: Fecal culture for aerobic pathogens of gastroenteritis. If kept sterile and properly sealed, Cary-Blair transport medium is stable in storage for several months and does not require refrigeration (before use and once inoculated).

Provides information to assist in interpretation of the test results, Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances, Recommendations for in-depth reading of a clinical nature, Customized Instructions & Shipping Guides. In areas where a cholera outbreak has not been declared: Any patient 2 years old or older presenting with acute watery diarrhea and severe dehydration or dying from acute watery diarrhea. A thorough patient history covering symptoms, severity and duration of illness, age, travel history, food consumption, history of recent antibiotic use, and illnesses in the family or other contacts will help the physician determine the appropriate testing to be performed. May include disease information, patient result explanation, recommendations, details of testing, associated diseases, explanation of possible patient results. © 1995–2020 Mayo Foundation for Medical Education and Research. Isolation and identification of Vibrio cholerae serogroup O1 or O139 by culture of a stool specimen remains the gold standard for the laboratory diagnosis of cholera. In areas where a cholera outbreak is declared: any person presenting with or dying from acute watery diarrhea. If an interpretive report is provided, the reference value field will state this. Therefore, a new plating medium, cellobiose-polymyxin B-colistin agar, was developed for … The codes reflect our interpretation of CPT coding requirements based upon AMA guidelines published annually. In the United States, Vibrio parahemolyticus is the most common cause of Vibrio disease. Thiosulfate-citrate-bile salts-sucrose agar has been routinely used for the isolation of pathogenic vibrios, although its selectivity for Vibrio cholerae and Vibrio vulnificus is inadequate.
The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website. Intervals are Mayo-derived, unless otherwise designated. The yield of Vibrio species is reduced when specimens are delayed in transit to the laboratory (>2 hours from collection for unpreserved specimen). It focuses on the epidemiology of cholera and the laboratory methods to isolate and identify Vibrio cholerae serogroups O1 and O139 and how to test their susceptibility to antimicrobial agents in the epidemic setting. Selective & Differential Media for Vibrio. While management of patients with acute watery diarrhea is similar regardless of the illness, it is important to identify cholera because of the potential for a widespread outbreak. Isolation and presumptive identification of agents from normally sterile sites 5. 1. Sixth edition. Delineates situations when tests are added to the initial order. No compliance statements are in use for this test. place a stool-soaked swab into Cary-Blair transport medium. Preserve 5 mL stool in enteric transport media (Cary-Blair) immediately (ARUP supply #29799). Indicates test has been approved by the New York State Department of Health. Therefore, it is recommended that fecal specimens that test positive for V. cholerae O1 and/or O139 by the Crystal® VC dipstick always be confirmed using traditional culture-based methods suitable for the isolation and identification of V. cholerae. Recommendations when to order or not order the test. In Clinical Microbiology Procedures Handbook, Fourth edition. The laboratory techniques and study methodology described provide accurate and … Thiosulfate citrate bile salts sucrose (TCBS) Agar is a selective agar for the isolation and cultivation of Vibrio cholerae and other Vibrio species. A suspected case with Vibrio cholerae O1 or O139 confirmed by culture or PCR and, in countries where cholera is not present or has been eliminated, the Vibrio cholerae O1 or O139 strain is demonstrated to be toxigenic. Mandell GL, Bennett JE, Dolin R: Principle's and Practice of Infectious Disease. Vibrio cholerae X.
Washington, DC, ASM Press, 2016, Section 3.8.1, 2. Appendices 1. (Min: 1 mL) ... Vibrio cholerae; Vibrio Culture; Stool Culture, Vibrio. Commercially available rapid test kits are useful in epidemic settings but do not yield an isolate for antimicrobial susceptibility testing and subtyping, and should not be used for routine diagnosis. WHO also recommends the following definition for confirmed cholera cases. Are you an ARUP Client? Collection and transport of sterile site specimens 4. May include intervals based on age and sex when appropriate. 87081; Identification CPT codes may vary based on method. Additional information related to the test. This manual is to be used for countries with minimal laboratory capacity and few resources.