The drop of blood should be in the center line, approximately ¼ inch from the frosted end. Follow these steps: There are two important guidelines to follow when submitting blood specimens. Use: Serum or clotted whole blood. There are four ways to donate: plasma, platelets, red cells, and whole blood.

Drop of blood too large or too small (usually too large). Puncture the diaphragm of the stopper by pushing the tube forward and initiating vacuum suction. Plasma Preparation. You will be subject to the destination website's privacy policy when you follow the link. Failure to push the spreader slide completely across the flat slide.

Place the tip of the pipette against the side of the tube, approximately ¼ inch above the cell layer. When plasma is required, follow these steps. The specified anticoagulant or preservative must be used for the test ordered. Prepare the second film in the same manner. Let go.

Also, never leave a tourniquet on the arm for more than one minute without releasing it.

Keep the spreader slide at a 45° angle, maintaining light but firm pressure with the spreader slide against the horizontal slide. concentrated granulocytes, lymphocytes, isolated mononuclear cells, and the Collect the venous blood in a vacuum tube containing anticoagulant (preferably EDTA); alternatively, collect the blood in a syringe and transfer it to a tube with anticoagulant; mix well. As soon as possible after collection, set the blood upright in a test tube rack. Plasma Preparation Using a Plasma Preparation Tube (PPT™). 8. (See Figure 7.) Also, print on the label the type of plasma submitted (eg, “Plasma, Sodium Citrate,” “Plasma, EDTA,” etc). The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website. If a frozen specimen is not required, it is not necessary to transfer serum to a plastic transport tube. When all samples have been drawn, remove the entire assembly from the arm. Please refer to the specimen requirements for the test(s) of interest available in the Directory of Services. Note: Please examine specimen collection and transportation supplies to be sure they do not include expired containers. 8.

The major blood cells are classified as red cells (erythrocytes), white cells (leukocytes), and platelets (thrombocytes). Frozen Gel Packs. (See Figure 5.) For additional information about blood collection, call the Division of Parasitic Diseases at (404) 718-4110. (See Figure 13. If the vacuum tube is not filling properly, and you are certain that you have entered the vein properly, substitute another tube.

The 4 types of Autologous Blood Collection study guide by Booguster includes 25 questions covering vocabulary, terms and more. (Water-soluble markers may wash off with freezing and transport.) Do not remove the stopper. Red-top tube: Contains no anticoagulant or preservative. Label all tubes clearly and carefully with all pertinent information or bar code.

We supply Blood collection series products, including Vacuum blood collection If the specimen cannot be properly collected, select another site and using new, sterile collection equipment, collect the specimen. Follow the manufacturer's recommendations on properly setting the needle. Do not reuse needles. This can lead to changes in concentration of certain analytes. Frozen Serum. All tubes should be labeled with the patient's full name or identification number as it appears on the test request form or affix bar code. No portion of the film should extend to the edges of the slide.

Activate the needle's safety feature and dispose of the needle in a sharps container.

Materials for proper specimen collection and transport are supplied by LabCorp. 9. Be sure to use only tubes with a 3.2% sodium citrate concentration. Green-top tube: Contains sodium heparin or lithium heparin. Slide preparation using venous blood is described below. comes from the vein.

7. When drawing blood, please follow all venipuncture procedures recommended for use by recognized organizations and/or in accordance with applicable state regulations involving phlebotomy practices. For after-hours pickup, follow the steps under Frozen Serum above. During and after a donation, we are able to separate those components, to give a recipient exactly what they need.

Prick the side of the pulp of the 3rd or 4th finger (alternate sites include ear lobe, or in infants large toe or heel). For some tests, such as chemistry procedures, fasting samples are often the specimen of choice. Prepare the film immediately, as soon as the drop of blood has been placed on the slide. When the slide contacts the drop, the blood will start to spread to the edges of the spreader slide. Failure to fill the tube will cause an improper blood-to-anticoagulant ratio and may yield questionable and/or QNS test results. the earlobe, fingertips, sometimes children with big toe or heel. The aseptic method of collecting and transporting a blood specimen works on the principle of a vacuum tube for drawing blood.

Both anticoagulants and preservatives may be required for plasma specimens. Butterfly needles often come with flexible tubing, whereas straight needles connect to a vacuum container and a syringe. To avoid clotting, mix the blood with the anticoagulant or preservative immediately after drawing each sample. For whole blood collection. Intravenous whole blood: The most widely used whole blood blood sample The essential difference between plasma and serum is that plasma retains fibrinogen (the clotting component), which is removed from serum. Always consult the test description and AccuDraw® prior to collection.

A separate frozen sample must be submitted for each test requiring a frozen specimen. 5. If blood is drawn for routine hematology, prepare the blood films (blood smears) immediately after collection. Label the tube carefully and clearly with all pertinent information or bar code. Collection Tube. ), 11. The blood should not extend past 3/4 the length of the slide.

If PCR is required, please refer to the molecular diagnosis section for appropriate blood collection procedures. To ensure accurate test results, all tubes containing an anticoagulant or preservative must be allowed to fill completely. Use: ACD whole blood. Excessive bleeding (longer than five minutes) should be brought to the attention of the physician. Immediately after removing the needle from the vein, gently touch the tip of the needle to one of the clean slides, producing a small drop of blood about 1 to 2 mm in diameter, about the size of a match head. Whenever possible, specimens should be collected before treatment is initiated. Label collection tubes and pre-cleaned slides (preferably frosted-end) with the patient’s name (or other identifier) and date and time of collection. : West 3F, Bldg 5, No.